Composite

Part:BBa_K5062048:Design

Designed by: Christian Nelson Tejedor Vicera   Group: iGEM24_HKU-HongKong   (2024-09-28)


CAR_Ma Mutliplexed shRNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2092
    Illegal EcoRI site found at 10240
    Illegal SpeI site found at 2040
    Illegal SpeI site found at 8557
    Illegal PstI site found at 4413
    Illegal PstI site found at 7716
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2092
    Illegal EcoRI site found at 10240
    Illegal NheI site found at 5284
    Illegal NheI site found at 6393
    Illegal NheI site found at 7210
    Illegal SpeI site found at 2040
    Illegal SpeI site found at 8557
    Illegal PstI site found at 4413
    Illegal PstI site found at 7716
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2092
    Illegal EcoRI site found at 10240
    Illegal BglII site found at 2214
    Illegal BamHI site found at 2076
    Illegal BamHI site found at 2439
    Illegal BamHI site found at 7347
    Illegal XhoI site found at 5448
    Illegal XhoI site found at 5509
    Illegal XhoI site found at 5550
    Illegal XhoI site found at 12326
    Illegal XhoI site found at 12441
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2092
    Illegal EcoRI site found at 10240
    Illegal SpeI site found at 2040
    Illegal SpeI site found at 8557
    Illegal PstI site found at 4413
    Illegal PstI site found at 7716
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2092
    Illegal EcoRI site found at 10240
    Illegal SpeI site found at 2040
    Illegal SpeI site found at 8557
    Illegal PstI site found at 4413
    Illegal PstI site found at 7716
    Illegal NgoMIV site found at 1603
    Illegal NgoMIV site found at 2881
    Illegal AgeI site found at 4989
    Illegal AgeI site found at 5358
    Illegal AgeI site found at 7353
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 975
    Illegal BsaI site found at 5547
    Illegal BsaI site found at 5565
    Illegal BsaI site found at 8312
    Illegal BsaI.rc site found at 2344
    Illegal BsaI.rc site found at 2991
    Illegal BsaI.rc site found at 5074
    Illegal BsaI.rc site found at 7817
    Illegal BsaI.rc site found at 9524
    Illegal BsaI.rc site found at 12118
    Illegal SapI.rc site found at 9729


Design Notes

This should be placed after a strong promoter on a vector created for in vitro transcription


Source

This was either manually designed, created with tools, found from genomic sequences, or from databases.

References